Antimicrobial Activity an Physicochemical Characterization of Extracts and Fractions of Rosmarinus officinalis and Origanum vulgare
DOI:
https://doi.org/10.21664/2238-8869.2022v11i1.p8-30Keywords:
antimicrobial activity, Origanum vulgare, plants extracts, Rosmarinus officinalisAbstract
Rosmarinus officinalis L. (Lamiaceae) is a shrubby woody with bittersweet taste, used in chips, broths, desserts, cookies, jams, fruit salads, and marmalades. Origanum vulgare L. (Lamiaceae) is a small plant used in meats, salads, soups, rice, potatoes and boiled eggs, as well as for perfuming and flavoring oils. Both have medicinal and aromatic properties. The aims of this study were: characterize, standardize herbal drugs, crude ethanol extracts and concentrated extracts of R. officinalis and O. vulgare, determine the polyphenols, tannins, flavonoids content; quantify the rosmarinic acid; evaluate the antimicrobial activity of rosmarinic acid, extracts, fractions of R. officinalis and O. vulgare against foodborne and clinical strains. The antimicrobial activity was evaluated by the broth microdilution method. The hexane and dichloromethane fractions of R. officinalis and O. vulgare and rosmarinic acid exhibited good inhibitory activity against some Gram-positive bacteria and food isolates. Ethyl acetate fraction of R. officinalis, concentrated extract, and fractions of O. vulgare and rosmarinic acid presented good inhibitory activity against Cryptococcus. These fractions have promising potential in the control of food pathogens and they can be used in the future as an alternative natural preservative in the industry.
References
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Do TKT, Hadji-Minaglou F, Antoniotti S, Fernandez X 2014. Secondary metabolites isolation in natural products chemistry: Comparison of two semi preparative chromatographic techniques (high-pressure liquid chromatography and high performance thin-layer chromatography). J Chromatogr A 1325: 256-260.
Erkan N, Ayranci G, Ayranci E 2008. Antioxidant activities of rosemary (Rosmarinus Officinalis L.) extract, blackseed (Nigella sativa L.) essential oil, carnosic acid, rosmarinic acid and sesamol. Food Chem 110:76-82.
Falco E, Mancini E, Roscigno G, Mignola E, Taglialatela-Scafati O, Senatore F 2013. Chemical composition and biological activity of essential oils of Origanum vulgare L. subsp. vulgare L. under different growth conditions. Molecules 18: 14948-14960.
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Frescura VS, Boligon AA, Athayde ML, Andriolo JL, Tedesco SB 2013. Compostos fenólicos em extratos de Rosmarinus Officinalis L. sob cultivo fora do solo. Enciclopédia Biosfera, Centro Científico Conhecer – Goiânia. 9: 755-761.
Gachkar L, Yadegari D, Rezaei MB, Taghizadeh M, Astaneh SA, Rasooli I 2007. Chemical and biological characteristics of Cuminum cyminum and Rosmarinus officinalis essential oils. Food Chem 102: 898 904.
Gohari AR, Saeidnia S, Shahverdi AR, Yassa N, Malmir M, Mollazade K, Naghinejad AR 2009. Phytochemistry and antimicrobial compounds of Hymenocrater calycinus. Eur Asian J Bio Sciences. 3: 64-68.
Gosslau A, Li S, Ho C, Chen KY, Rawson NE 2011. The importance of natural product characterization in studies of their anti-inflammatory activity. Mol Nutr Food Res 55: 74-82.
Grevsen K, Fretté XC, Christensen LP 2009. Content and composition of volatile terpenes, flavonoids and phenolic acids in greek oregano (Origanum vulgare L. ssp. hirtum) at different development stages during cultivation in cool temperate climate. Eur J Hortic Sci 74: 193-203.
Holetz FB, Pessini GL, Sanches NR, Cortez DAG, Nakamura CV, Dias Filho BP 2002. Screening of some plants used in the Brazilian folk medicine for the treatment of infectious diseases. Mem Inst Oswaldo Cruz 97: 1027-1031.
Janampa E, Perez H, Mauricio F, Alvítez‑Temoche D, Mauricio‑Vilchez C, Gallo W, Mayta‑Tovalino F 2020. Antibacterial activity of ethanol extract of the leaves of Rosmarinus officinalis L. at different concentrations versus Streptococcus mutans: An in vitro comparative study. European J Gen Dent 9: 152-156.
Kadariya J, Smith TC, Thapaliya D 2014. Staphylococcus aureus and Staphylococcal food-borne disease: an ongoing challenge in public health. Bio Med Res Int 2014:1-9.
Kaurinovic B, Popovic M, Vlaisavljevic S, Trivic S. 2011. Antioxidant capacity of Ocimum basilicum L. and Origanum vulgare L. extracts. Molecules 16: 7401-7414.
Kontoyiannis DP, Vaziri I, Hanna HA, Boktour M, Thornby J, Hachem R, Bodey GP, Raad II 2001. Risk factors for Candida tropicalis fungemia in patients with cancer. Clin Infectious Dis 33: 1676-1681.
Kruppa PC, Russomanno OMR 2008. Ocorrência de fungos em sementes de plantas medicinais, aromáticas e condimentares da família Lamiaceae. Tropical Plant Patholgy 33: 45–51.
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2022-04-29
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CHAUL, Luiza Toubas; ALVES, Virgínia Farias; SÁ, Stone de; OLIVEIRA, Leandra de Almeida Ribeiro; FIUZA, Tatiana de Sousa; TORRES, Ieda Maria Sapateiro; CONCEIÇÃO, Edemilson Cardoso da; PAULA, José Realino de. Antimicrobial Activity an Physicochemical Characterization of Extracts and Fractions of Rosmarinus officinalis and Origanum vulgare. Fronteiras - Journal of Social, Technological and Environmental Science, [S. l.], v. 11, n. 1, p. 8–30, 2022. DOI: 10.21664/2238-8869.2022v11i1.p8-30. Disponível em: https://revistas2.unievangelica.edu.br/index.php/fronteiras/article/view/4029. Acesso em: 23 nov. 2024.
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