Morphoanatomic Study and Antibacterial Activity of Essential Oil, Crude Extract and Fractions of Macairea radula (Bonpl.) Dc. Leaves
DOI:
https://doi.org/10.21664/2238-8869.2020v9i1.p499-523Keywords:
Antimicrobial Activity, Cerrado, Volatile Oil, Medicinal PlantsAbstract
Macairea radula, “capuchinha”, is a shrub popularly used for dermatoses and scabies. The aim of this study was to perform the morpho-anatomical study, the phytochemical screening, the antibacterial activity of essential oil, crude extract and leaves fractions. The macroscopic characterization was carried out in the Serra dos Pireneus, Goiás. Anatomical studies and phytochemical screening were performed according to conventional techniques. The antibacterial activity was determined through the Minimum Inhibitory Concentration. The essential oil was obtained by hydrodistillation in a Clevenger apparatus. M. radula has simple, opposite leaves. The leaf blade is hypoestomatic, trichomes on both faces. It was observed coumarins, steroids, triterpenes, tannins, deoxy sugars, flavonoid and saponin. Antimicrobial activity of the crude extract and aqueous fraction against Pseudomonas aeruginosa, dichloromethane and ethyl acetate fractions against Listeria innocua were observed. The results open future perspectives for its use in the development of products with potential antibacterial activity.
References
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Hein, Ingeborg, Dieter Klein, Angelika Lehner, Andreas Bubert, Ernst Brandl, and Martin Wagner. 2001. “Detection and Quantification of the Iap Gene of Listeria Monocytogenes and Listeria Innocua by a New Real-Time Quantitative PCR Assay.” Research in Microbiology 152 (1): 37–46. https://doi.org/10.1016/S0923-2508(00)01166-9.
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Holetz, Fabíola Barbiéri, Greisiele Lorena Pessini, Neviton Rogério Sanches, Diógenes Aparício Garcia Cortez, Celso Vataru Nakamura, and Benedito Prado Dias Filho. 2002. “Screening of Some Plants Used in the Brazilian Folk Medicine for the Treatment of Infectious Diseases.” Memorias Do Instituto Oswaldo Cruz 97 (7): 1027–31. https://doi.org/10.1590/S0074-02762002000700017.
Johansen, Donald. 1940. Plant Microtechnique. 1st editio. New York ;;London: McGraw-Hill Book Company Inc.
Keating, G.J., and R. O’Kennedy. 1997. “The Chemistry and Occurrence of Coumarins.” In Coumarins : Biology, Applications, and Mode of Action, 348. Wiley.
Keating, Richard C. 1984. “Leaf Histology and Its Contribution to Relationships in the Myrtales.” Annals of the Missouri Botanical Garden 71 (3): 801. https://doi.org/10.2307/2399163.
Liu, Shaowei, Virenda M. Puri, and Ali Demirci. 2009. “Evaluation of Listeria Innocua as a Suitable Indicator for Replacing Listeria Monocytogenes during Ripening of Camembert Cheese.” International Journal of Food Science & Technology 44 (1): 29–35. https://doi.org/10.1111/j.1365-2621.2007.01629.x.
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Pieroni, Laís Goyos, Fernanda Mendes de Rezende, Valdecir Farias Ximenes, and Anne Lígia Dokkedal. 2011. “Antioxidant Activity and Total Phenols from the Methanolic Extract of Miconia Albicans (Sw.) Triana Leaves.” Molecules 16 (11): 9439–50. https://doi.org/10.3390/molecules16119439.
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Scalbert, Augustin. 1991. “Antimicrobial Properties of Tannins.” Phytochemistry 30 (12): 3875–83. https://doi.org/10.1016/0031-9422(91)83426-L.
Schuchat, Anne, Katherine A. Deaver, Jay D. Wenger, Brian D. Plikaytis, Laurene Mascola, Robert W. Pinner, Arthur L. Reingold, and Claire V. Broome. 1992. “Role of Foods in Sporadic Listeriosis: I. Case-Control Study of Dietary Risk Factors.” JAMA: The Journal of the American Medical Association 267 (15): 2041–45. https://doi.org/10.1001/jama.1992.03480150047035.
Shorthouse, Joseph D., and Odette. Rohfritsch. 1992. “Diversity of Gall-Inducing Insects and Their Galls.” In Biology of Insect-Induced Galls, 285. Oxford University Press.
Silva, Marina Aparecida De Oliveira E, and Rosana Romero. 2008. “Melastomataceae from Mountain of the Municipality of the Delfinópolis, Minas Gerais, Brazil.” Rodriguesia 59 (4): 609–47. https://doi.org/10.1590/2175-7860200859401.
Solereder, Hans. 1908. Systematic Anatomy Of The Dicotyledons. https://archive.org/details/in.ernet.dli.2015.217381.
Somavilla, Nádia Sílvia, and Dalva Graciano-Ribeiro. 2011. “Análise Comparativa Da Anatomia Foliar de Melastomataceae Em Ambiente de Vereda e Cerrado Sensu Strict.” Acta Botanica Brasilica 25 (4): 764–75. https://doi.org/10.1590/S0102-33062011000400004.
Souza, Rosangela Cristina Occhi Sampaio De, and Osnir Marquete. 2000. “Miconia Tristis Spring e Miconia Doriana Cogn. (Melastomataceae): Anatomia Do Eixo Vegetativo e Folhas.” Rodriguesia 51 (78–79): 133–42. https://doi.org/10.1590/2175-7860200051787905.
Stern, J. Lewis, Ann E. Hagerman, Peter D. Steinberg, and Pamela K. Mason. 1996. “Phlorotannin-Protein Interactions.” Journal of Chemical Ecology 22 (10): 1877–99. https://doi.org/10.1007/BF02028510.
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Ventura, Claiton Pires, Alaíde Braga de Oliveira, and Fernão Castro Braga. 2007. “Antimicrobial Activity of Trembleya Laniflora, Xyris Platystachia and Xyris Pterygoblephara.” Brazilian Journal of Pharmacognosy 17 (1): 17–22. https://doi.org/10.1590/S0102-695X2007000100005.
Alves, Virgínia, Ana Clara Melo Lima, Luiza Chaul Toubas, Aline Neves Pereira, Geovanna Bárbara henrique correia da Silva, and Ieda Maria sapateiro Torres. 2015. “Pesquisa de Listeria Monocytogenes Em Queijo Muçarela Fatiado Comercializado Em Estabelecimentos Varejistas Na Cidade de Goiânia, GO.” Revista Eletrônica de Farmácia 12 (4). https://doi.org/10.5216/ref.v12i4.38500.
Baider, C., M. Tabarelli, and W. Mantovani. 2001. “The Soil Seed Bank during Atlantic Forest Regeneration in Southeast Brazil.” Revista Brasleira de Biologia 61 (1): 35–44. https://doi.org/10.1590/s0034-71082001000100006.
Baumgratz, F. 1972. “Bermer Kungenzur Doppel Farbung Astrablau-Safranin.” Mikrokosmos 61.
Baumgratz, José Fernando A. 2009. “Macairea.” 2009. http://floradobrasil.jbrj.gov.br/reflora/listaBrasil/ConsultaPublicaUC/BemVindoConsultaPublicaConsultar.do?invalidatePageControlCounter=1&idsFilhosAlgas=%5B2%5D&idsFilhosFungos=%5B1%2C10%2C11%5D&lingua=&grupo=5&familia=null&genero=Macairea&especie=radula&.
Baumgratz, José Fernando A., and Geisa Lauro Ferreira. 1980. “Estudo Da Nervação e Epiderme Foliar Das Melastomataceae Do Município Do Rio de Janeiro. Gênero Miconia. Seção Miconia.” Rodriguesia 32 (54): 161–69. https://doi.org/10.1590/2175-78601980325411.
Brasil. 2010. “Farmacopeia Brasileira - Agência Nacional de Vigilância Sanitária & Fundação Oswaldo Cruz.” www.anvisa.gov.br.
Carneiro, Marco Antonio A., Rafael A.X. Borges, Ana P.A. Araújo, and G. Wilson Fernandes. 2009. “Insetos Indutores de Galhas Da Porção Sul Da Cadeia Do Espinhaço, Minas Gerais, Brasil.” Revista Brasileira de Entomologia 53 (4): 570–92. https://doi.org/10.1590/s0085-56262009000400007.
Celotto, Andréa Carla, Daniela Zaupa Nazario, Marcela De Almeida Spessoto, Carlos Henrique Gomes Martins, and Wilson Roberto Cunha. 2003. “Evaluation of the in Vitro Antimicrobial Activity of Crude Extracts of Three Miconia Species.” Brazilian Journal of Microbiology 34 (4): 339–40. https://doi.org/10.1590/S1517-83822003000400010.
Chan, Wilfred R., Veronica Sheppard, Kathleen A. Medford, Winston F. Tinto, William F. Reynolds, and Stewart McLean. 1992. “Triterpenes from Miconia Stenostachya.” Journal of Natural Products 55 (7): 963–66. https://doi.org/10.1021/np50085a020.
CLSI, Clinical and Laboratory Standards Institute. 2005. “Methods for Dilution Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria; Proposed Guideline.”
———. 2012. “Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically.” www.clsi.org.
Costa. 1977. “Ano XXIX-Número 43.” In Rodriguesia Ano XXIX — Número 43, 297.
Costa, A. F. 2001. Farmacognosia. Fundação Calouste Gulbenkian.
Cunha, A. Proença da. 2005. Farmacognosia e Fitoquímica. Fundação Calouste Gulbenkian. Serviço de Educação e Bolsas.
Farias, M.R. 2004. “Farmacognosia Da Planta Ao Medicamento.” In Avaliação Da Qualidade de Matérias-Primas Vegetais, 263–88. https://www.saraiva.com.br/farmacognosia-da-planta-ao-medicamento-5-ed-2004-160826/p.
Fernandes, Sarah Rodrigues, Heleno Dias Ferreira, Luiza Toubas Chaul, Pierre Alexandre Dos Santos, Virgínia Farias Alves, Leonice Manrique Faustino Tresvenzol, Leonardo Luiz Borges, José Realino De Paula, and Tatiana Souza Fiuza. 2016. “Estudo Anatômico de Trembleya Phlogiformis DC.” Revista Eletrônica de Farmácia 13 (2): 89. https://doi.org/10.5216/ref.v13i2.41236.
Ferri, P.H. 1996. “Química de Produtos Naturais: Métodos Gerais.” In Plantas Medicinais Arte e Ciências. Um Guia de Estudo Interdisciplinar, 230. https://www.editorametha.com.br/livros-de-nutricao/fitoterapia/plantas-medicinais-arte-e-ci-ncia-um-guia-de-estudo-interdisciplinar.html.
Fessenden, Ralph J., and Joan S. Fessenden. 1982. Organic Chemistry. https://openlibrary.org/books/OL4267488M/Organic_chemistry.
Gilbert, Ateufack, Tadjoua Tchoumbou Herve, Yousseu Nana William, Sama Fonkeng Leonard, Kuiate Jules-Roger, and Kamanyi Albert. 2014. “Antidiarrhoeal and Antibacterial Activity of Aqueous and Methanolic Leaves Extracts of Dissotis Thollonii Cogn. (Melastomataceae).” Asian Pacific Journal of Tropical Biomedicine 4: S672–78. https://doi.org/10.12980/APJTB.4.201414B214.
Hein, Ingeborg, Dieter Klein, Angelika Lehner, Andreas Bubert, Ernst Brandl, and Martin Wagner. 2001. “Detection and Quantification of the Iap Gene of Listeria Monocytogenes and Listeria Innocua by a New Real-Time Quantitative PCR Assay.” Research in Microbiology 152 (1): 37–46. https://doi.org/10.1016/S0923-2508(00)01166-9.
Hofer, Ernesto, Rejane Soares do Nascimento, and Marta Antunes de Oliveira. 1998. “Meningite Por Listeria Monocytogenes. Relato de Casos Em Pacientes Do Distrito Federal Meningitis Due to Listeria Monocytogenes. Case Reports in Patients of Distrito Federal, Brazil.” Revista Da Sociedade Brasileira de Medicina Tropica 31 (2): 173–77.
Holetz, Fabíola Barbiéri, Greisiele Lorena Pessini, Neviton Rogério Sanches, Diógenes Aparício Garcia Cortez, Celso Vataru Nakamura, and Benedito Prado Dias Filho. 2002. “Screening of Some Plants Used in the Brazilian Folk Medicine for the Treatment of Infectious Diseases.” Memorias Do Instituto Oswaldo Cruz 97 (7): 1027–31. https://doi.org/10.1590/S0074-02762002000700017.
Johansen, Donald. 1940. Plant Microtechnique. 1st editio. New York ;;London: McGraw-Hill Book Company Inc.
Keating, G.J., and R. O’Kennedy. 1997. “The Chemistry and Occurrence of Coumarins.” In Coumarins : Biology, Applications, and Mode of Action, 348. Wiley.
Keating, Richard C. 1984. “Leaf Histology and Its Contribution to Relationships in the Myrtales.” Annals of the Missouri Botanical Garden 71 (3): 801. https://doi.org/10.2307/2399163.
Liu, Shaowei, Virenda M. Puri, and Ali Demirci. 2009. “Evaluation of Listeria Innocua as a Suitable Indicator for Replacing Listeria Monocytogenes during Ripening of Camembert Cheese.” International Journal of Food Science & Technology 44 (1): 29–35. https://doi.org/10.1111/j.1365-2621.2007.01629.x.
Macari, P.A.T., V. de P. Emerenciano, and Z.M.G.S. Ferriera. 1990. “Identification of Triterpenes from Miconia Albicans through Analysis by Microcomputer.” Quim Nova 13: 260–62.
Metcalfe, C., and L. Chalk. 1950. Anatomy of the Dicotyledons : Leaves, Stem, and Wood in Relation to Taxonomy, with Notes on Economic Uses. Oxford: Clarendon Press.
Mors, W. B., C. T. Rizzini, and N. A. Pereira. 2000. Medicinal Plants of Brazil. Reference Publications, Inc. Reference Publications, Inc.
Murray, P., K.S. Rosenthal, and M.A. Pfaller. 2011. Microbiologia Médica. https://www.saraiva.com.br/microbiologia-medica-6-ed-3097124/p.
O’Kennedy, R. Richard, and R. D. Robert Douglas Thornes. 1997. Coumarins : Biology, Applications, and Mode of Action. Wiley.
Pieroni, Laís Goyos, Fernanda Mendes de Rezende, Valdecir Farias Ximenes, and Anne Lígia Dokkedal. 2011. “Antioxidant Activity and Total Phenols from the Methanolic Extract of Miconia Albicans (Sw.) Triana Leaves.” Molecules 16 (11): 9439–50. https://doi.org/10.3390/molecules16119439.
Razera, Fernanda, Gislaine Silveira Olm, and Renan Rangel Bonamigo. 2011. “Dermatoses Neutrofílicas - Parte II.” An Bras Dermatol 86 (2): 195–211.
Santos, Andrea Karla A., Angela Borges Martins, Rosana Romero, Ana Paula M. Santos, Frank Almeda, Karina Fidanza Rodrigues Bernado, Cristiana Koschnitzke, et al. 2009. “Melastomataceae.” In Plantas Raras Do Brasil, 263–79.
Scalbert, Augustin. 1991. “Antimicrobial Properties of Tannins.” Phytochemistry 30 (12): 3875–83. https://doi.org/10.1016/0031-9422(91)83426-L.
Schuchat, Anne, Katherine A. Deaver, Jay D. Wenger, Brian D. Plikaytis, Laurene Mascola, Robert W. Pinner, Arthur L. Reingold, and Claire V. Broome. 1992. “Role of Foods in Sporadic Listeriosis: I. Case-Control Study of Dietary Risk Factors.” JAMA: The Journal of the American Medical Association 267 (15): 2041–45. https://doi.org/10.1001/jama.1992.03480150047035.
Shorthouse, Joseph D., and Odette. Rohfritsch. 1992. “Diversity of Gall-Inducing Insects and Their Galls.” In Biology of Insect-Induced Galls, 285. Oxford University Press.
Silva, Marina Aparecida De Oliveira E, and Rosana Romero. 2008. “Melastomataceae from Mountain of the Municipality of the Delfinópolis, Minas Gerais, Brazil.” Rodriguesia 59 (4): 609–47. https://doi.org/10.1590/2175-7860200859401.
Solereder, Hans. 1908. Systematic Anatomy Of The Dicotyledons. https://archive.org/details/in.ernet.dli.2015.217381.
Somavilla, Nádia Sílvia, and Dalva Graciano-Ribeiro. 2011. “Análise Comparativa Da Anatomia Foliar de Melastomataceae Em Ambiente de Vereda e Cerrado Sensu Strict.” Acta Botanica Brasilica 25 (4): 764–75. https://doi.org/10.1590/S0102-33062011000400004.
Souza, Rosangela Cristina Occhi Sampaio De, and Osnir Marquete. 2000. “Miconia Tristis Spring e Miconia Doriana Cogn. (Melastomataceae): Anatomia Do Eixo Vegetativo e Folhas.” Rodriguesia 51 (78–79): 133–42. https://doi.org/10.1590/2175-7860200051787905.
Stern, J. Lewis, Ann E. Hagerman, Peter D. Steinberg, and Pamela K. Mason. 1996. “Phlorotannin-Protein Interactions.” Journal of Chemical Ecology 22 (10): 1877–99. https://doi.org/10.1007/BF02028510.
Terras, Franky R.G., Hilde M.E. Schoofs, Karin Thevissen, Rupert W. Osborn, Jozef Vanderleyden, Bruno P.A. Cammue, and Willem F. Broekaert. 1993. “Synergistic Enhancement of the Antifungal Activity of Wheat and Barley Thionins by Radish and Oilseed Rape 2S Albumins and by Barley Trypsin Inhibitors.” Plant Physiology 103 (4): 1311–19. https://doi.org/10.1104/pp.103.4.1311.
Torssell, Kurt. 1983. Natural Product Chemistry : A Mechanistic and Biosynthetic Approach to Secondary Metabolism. Wiley.
Ventura, Claiton Pires, Alaíde Braga de Oliveira, and Fernão Castro Braga. 2007. “Antimicrobial Activity of Trembleya Laniflora, Xyris Platystachia and Xyris Pterygoblephara.” Brazilian Journal of Pharmacognosy 17 (1): 17–22. https://doi.org/10.1590/S0102-695X2007000100005.
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2020-03-05
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TOMÉ, Luciana Uchôa; FERREIRA, Heleno Dias; OLIVEIRA, Larissa Gonçalves de; ALVES, Virgínia Farias; PAULA, José Realino de; FARIAS, Wadson da Costa; BORGES, Leonardo Luiz; FIUZA, Tatiana de Sousa. Morphoanatomic Study and Antibacterial Activity of Essential Oil, Crude Extract and Fractions of Macairea radula (Bonpl.) Dc. Leaves. Fronteiras - Journal of Social, Technological and Environmental Science, [S. l.], v. 9, n. 1, p. 499–523, 2020. DOI: 10.21664/2238-8869.2020v9i1.p499-523. Disponível em: https://revistas2.unievangelica.edu.br/index.php/fronteiras/article/view/2796. Acesso em: 22 nov. 2024.
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