Study of Oxidative Profile of Transgenic Beans by Means of the HR-MAS NMR

Authors

  • Erick de Oliveira Lemes Faculdade Anhanguera de Anápolis.
  • Rafael Choze Universidade Federal de Goiás e Faculdade Anhanguera de Anápolis/GO.
  • Joel Rocha da Silva Universidade Federal de Goiás – UFG.
  • Daiana da Silva Vargem Faculdade Anhanguera de Anápolis.

DOI:

https://doi.org/10.21664/2238-8869.2015v4i2.p194-204

Abstract

In Brazil, beans are one of the most important food legume, is considered the main source of proteins in low-income populations. One of the major diseases affecting the common bean golden mosaic is transmitted by the whitefly, by BGMV viruses (Bean Golden Mosaic Virus). Thus, EMBRAPA rice and beans developed a genotype resistant to BGMV called Olathe 5.1. Two other genetically modified strains were produced (5.1 Treasury and Ivory 5.1). The 1H NMR spectra HR-MAS led to the structures of the flavonoids quercetin and myricetin, present in higher concentration in genetically modified beans. Thus a study of the oxidative profile of these cultivars, where the flavonoid kaempferol has undergone a change in its content due to exposure to these genotypes physical uncontrolled conditions was conducted.

Keywords: Flavonoids; HR-MAS; Chemometrics.

Author Biographies

Erick de Oliveira Lemes, Faculdade Anhanguera de Anápolis.

Graduado em Farmácia pela Faculdade Anhanguera de Anápolis.

Rafael Choze, Universidade Federal de Goiás e Faculdade Anhanguera de Anápolis/GO.

Doutor em Química Orgânica (RMN) pela Universidade Federal de Goiás. Docente na Universidade Federal de Goiás e Pesquisador da Faculdade Anhanguera de Anápolis/GO.

Joel Rocha da Silva, Universidade Federal de Goiás – UFG.

Mestre em Química pela Universidade Federal de Goiás – UFG.

Daiana da Silva Vargem, Faculdade Anhanguera de Anápolis.

Mestre em Ciências Ambientais pelo Centro Universitário de Anápolis – UniEVANGÉLICA. Coordenadora de curso na Faculdade Anhanguera de Anápolis.

References

Antignus Y, Vunsh R, Lachman O, Pearlsman M, Maslnin L, Hanany U, Rosner A 2004. Truncanted Rep gene originated from Tomato yellow leaf curl virus-Israel (Mild) confers strain-specific resistance in transgenicc tomato. Annals of Applied Biology, 144. p. 39.
Asad S, Haris WA, Bashir A, Zafar Y, Malik KA, Malik NN, Lichtenstein CP 2003. Transgenic tobacco expressing geminiviral RNAs are resistant to the serious viral pathogen causing cotton leaf curl disease. Archives of Virology, 148. p. 2341.
Brunetti A, Tavazza M, Noris E, Tavazza R, Caciagli P, Ancora G, CRESPI S, Accotto GP 1997. High expression of truncated viral Rep protein confers resistance to Tomato yellow leaf curl virus in transgenic tomato plants. Molecular Plant-Microbe Interactics, 10:571-579.
Cardador-Martínez A, Loarca-Piña G, Oomah BD 2002. Antioxidant activity in common beans (Phaseolus vulgaris L.). Journal of Agricultural Food Chemistry, 50. p. 6975.
Chellappan P, Masona MV, Vanitharani R, Taylor N, Fauquet C 2004. Broad spectrum resistance to ssDNA viruses associated with transgene-induced gene silencing in cassava. Plant Molecular Biology, 56. p. 601.
Duan YP, Powell CA, Purcifull DE, Broglio P, Hiebert E 1997. Phenotypic variation in transgenic tobacco expressing mutated geminivirus movement/pathogenicity (BC1) proteins. Molecular Plant-Microbe Interactics, 10. p. 1065.
Lin L, Harnly JM, Pastor-Corrales MS, Luthria DL 2008. The polyphenolic profiles of common bean (Phaseolus vulgaris L.). Food Chemistry, 107. p. 399.
Manly BJF 2008. Métodos estatísticos multivariados: uma introdução. Porto Alegre: Bookman. 2008.
Perazzini R, Leonardi D, Ruggeri S, Alesiani D, D’Arcangelo A, Canini A 2008. Characterization of Phaseolus vulgaris L. Landraces Cultivated in Central Italy. Plant Foods for Human Nutrition, 63. p. 211.
Zhang P, Vanderschuren H, Futterer J, Gruissem W 2005. Resistance to cassava mosaic disease in transgenic cassava expressing antisense RNAs targeting virus replication genes. Plant Biotechnology Journal, 3. p. 385.

Published

2015-11-20

How to Cite

LEMES, Erick de Oliveira; CHOZE, Rafael; SILVA, Joel Rocha da; VARGEM, Daiana da Silva. Study of Oxidative Profile of Transgenic Beans by Means of the HR-MAS NMR. Fronteiras - Journal of Social, Technological and Environmental Science, [S. l.], v. 4, n. 2, p. 194–204, 2015. DOI: 10.21664/2238-8869.2015v4i2.p194-204. Disponível em: https://revistas2.unievangelica.edu.br/index.php/fronteiras/article/view/1346. Acesso em: 21 nov. 2024.

Issue

Section

National Symposium of Science and Environment (SNCMA2014)